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Antisense anti IGF-I cellular therapy of malignant tumours: Rational design of immunostimulatory siRNAs. These short molecules are supposed to be more specific than antisense RNA and devoid of undesired effects [11]. Statistical analyses were conducted using Prism 5.

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Maria Pia Silva Castro Professora. Protein Families. Select province Other Place of Birth: After 48 h, cells were inactivated by irradiation and then used for immunization of mice according to previous procedures Methods S1 [22].

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The presence of an immune response after down-regulation of IGF-IR using antisense RNA construct was also confirmed in a mouse neuroblastoma model [13]. When you are logged in as a myFreeOnes Member you will earn credits if you fill in this form! Studied at No. Indeed, plasmids or oligonucleotides carrying non-methylated CpG sequences are known to trigger innate immune responses and can develop strong toxicity in human cells [36] , [37].

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Cell-cycle analysis was done using propidium iodide 2. The hormone-dependent ductal tumor line C4HD was originated in mice treated with 40 mg medroxyprogesterone acetate MPA, medrosterona, Laboratorios Gador, Buenos Aires, Argentina every 3 months for 1 year, and has been maintained by serial transplantation in animals treated with 40 mg subcutaneously s. Comparisons among groups were performed with one-way analysis of variance test.